Interference of ferric ions with ferrous iron quantification using the ferrozine assay.

TitleInterference of ferric ions with ferrous iron quantification using the ferrozine assay.
Publication TypeJournal Article
Year of Publication2013
AuthorsIm J, Lee J, Löffler FE
JournalJ Microbiol Methods
Volume95
Issue3
Pagination366-7
Date Published2013 Dec
ISSN1872-8359
KeywordsChemistry Techniques, Analytical, Colorimetry, Darkness, Diagnostic Errors, Ferric Compounds, Ferrous Compounds, Ferrozine, Ions, Iron, Time Factors
Abstract

The ferrozine assay is a widely used colorimetric method for determining soluble iron concentrations. We provide evidence for a heretofore unrecognized interference of ferric ions (Fe(3+)) on ferrous iron (Fe(2+)) measurements performed in the dark. Fe(3+) concentrations affected the absorbance measurements, which linearly increased with incubation time.
DOI10.1016/j.mimet.2013.10.005
Alternate JournalJ. Microbiol. Methods
PubMed ID24140574
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